Turnaround times
The quoted turnaround time is from sample receipt in the laboratory, to results authorisation in the Laboratory Information Management system. The times do not include transport of specimen to the laboratory or the administrative process to print and post/email reports. Service users must allow for transport and reporting time when ordering tests.
Clinical background:
Useful in the investigation of Familial Haemophagocytic Lymphohistiocytosis (FHLH).
Specimen container paediatric:
2 EDTA Required – 1x Patient & 1x Control
Specimen container adult:
2 EDTA Required – 1x Patient & 1x Control
Minimum volume paediatric:
Both EDTA tubes – 4ml
Minimum volume adult:
Both EDTA tubes – 4ml
Special requirements:
The requesting clinician must arrange for a control sample from a healthy volunteer to arrive at the same time as the patient’s sample (please label ‘control’).
Samples should ideally be tested as fresh as possible. Keep at room temperature at all times. Samples should arrive in the laboratory no later than 13:00 to be processed the same day.
Transport requirements:
Please sent to Flow Cytometry, Blood Sciences Lab, RVI.
Freq analysis:
As required
Add on test:
Use the contact details below to discuss if an add-on is required
Interpretation:
None
Reference ranges:
Normal Result: Normal
Other info:
This is performed by stimulation with the K562 line +/- overnight incubation with IL-2 to activate the NK cells. Perforin expression should also be requested as per of FHLH investigation. If both are normal SAP and XIAP should be excluded in males. This test is only available by prior arrangement with the laboratory (Ext:25078) and discussion with a clinical immunologist (paediatric or adult). Samples should ideally be tested as fresh as possible. Keep at room temperature at all times. Samples should arrive in the laboratory no later than 13:00 to be processed on the same day.