Turnaround times
The quoted turnaround time is from sample receipt in the laboratory, to results authorisation in the Laboratory Information Management system. The times do not include transport of specimen to the laboratory or the administrative process to print and post/email reports. Service users must allow for transport and reporting time when ordering tests.
Clinical background:
Fluorescence in situ hybridisation (FISH) is generally carried out using commercial probe sets on interphase cells from short term cell cultures (e.g. bone marrow or blood) or on FFPE (formalin fixed paraffin embedded) sections or cells released from FFPE blocks or curls. FISH can identify specific gene fusion events such as the Philadelphia chromosome in CML (BCR::ABL1) . A wide range of FISH probes are available and will be performed at the discretion of a clinical scientist depending on the clinical details or can be requested by a consultant haematologist or histopathologist.
Specimen container paediatric:
Lithium heparin or EDTA for liquid samples and FFPE for tissue biopsies
Specimen container adult:
Lithium heparin or EDTA for liquid samples and FFPE for tissue biopsies
Minimum volume paediatric:
3ml
Minimum volume adult:
3ml
Special requirements:
FFPE slides alternative. Please complete NEHODS request form
Sample stability:
N/A
Transport requirements:
Samples should be sent to laboratory immediately.
Freq analysis:
Daily
Add on test:
Can be added to any genetics sample where cultured cells have been stored
Quality assurance:
UK NEQAS
Interpretation:
By genetics clinical scientist
Reference ranges:
N/A
Factors affecting result:
Quality and size of sample
Referenced documents:
N/A
Other info:
If an urgent result is required e.g. PML::RARA or BCR::ABL1 please contact the lab.