Flow cytometry is a technology that is used to analyse the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. Cell components are fluorescently labelled and then excited by the laser to emit light at varying wavelengths.
The fluorescence can be measured to determine various properties of single particles, which are usually cells. Thousands of particles per second can be analysed as they pass through the liquid stream. Examples of the properties measured include the relative size and granularity of the cell as well as its internal complexity and the intensity of the fluorescent signal. An optical-to-electronic coupling system is used to record the way in which the particle emits fluorescence and scatters incident light from the laser.
Flow cytometry is routinely used in the diagnosis of health disorders, especially blood cancers, but has many other applications in basic research, clinical practice and clinical trials. A common variation is to physically sort particles based on their properties, so as to purify populations of interest.
For external users, please download Flow Cytometry request forms here:
Please send samples to:
Flow Laboratory, Blood Sciences Level 3, Leazes Wing Royal Victoria Infirmary Richardson Road Newcastle upon Tyne NE1 4LP